CORE--CELL AND ANTIBODY CORE

$312,755P01FY2002CANIH

Wistar Institute, Philadelphia PA

Investigators

Linked publications & trials

Paper 36054930 Paper 34462276 Paper 34334830 Paper 31618628 Paper 30575721 Paper 30089911 Paper 30038220 Paper 29695835 Paper 29141225 Paper 29101162 Paper 28953887 Paper 28928360 Paper 28927893 Paper 28925403 Paper 28484090 Paper 28445987 Paper 28106104 Paper 27655717 Paper 27477320 Paper 27448973 Paper 27210749 Paper 27119099 Paper 27100738 Paper 27043285 Paper 26850518 Paper 26673799 Paper 26659798 Paper 26601857 Paper 26559571 Paper 26447206 Paper 26343386 Paper 26251218 Paper 26072686 Paper 26013381 Paper 25705850 Paper 25695690 Paper 25619837 Paper 25617424 Paper 25602801 Paper 25512635 Paper 25510211 Paper 25453510 Paper 25417704 Paper 25356520 Paper 25109840 Paper 25050081 Paper 24705312 Paper 24590809 Paper 24561508 Paper 24460190 Paper 24445538 Paper 24258995 Paper 24258984 Paper 24157461 Paper 24132921 Paper 24128326 Paper 24104062 Paper 23897276 Paper 23890088 Paper 23764003 Paper 23624919 Paper 23608752 Paper 23552384 Paper 23527225 Paper 23489578 Paper 23403634 Paper 23334329 Paper 22982443 Paper 22971921 Paper 22969117 Paper 22959031 Paper 22498258 Paper 22339359 Paper 22131348 Paper 21948558 Paper 21847077 Paper 21707960 Paper 21523820 Paper 21447722 Paper 21358674 Paper 21156289 Paper 20729912 Paper 20478252 Paper 20159965 Paper 20055909 Paper 19968818 Paper 19549918 Paper 19493000 Paper 19078957 Paper 19049876 Paper 19033657 Paper 18925393 Paper 18832450 Paper 18794803 Paper 18790768 Paper 18761657 Paper 18632627 Paper 18532874 Paper 18505964 Paper 18368129

Abstract

Cell lines and monoclonal antibodies (Mab) are indispensable tools for experimental investigations. This group has generated one of the largest cell banks of cultured melanocytes from normal skin, nevi, and primary and metastatic melanomas. The cell bank is continuously expanding due to the needs of all investigators of this program project. The objectives are to culture cells, characterize their phenotypes, freeze stocks and quality- control all cells regarding mycoplasma infections. Cultures are then distributed to all investigators within the program project and to other interested scientists. MAb are being produced for research projects using in vivo subtraction protocols to achieve a higher percentage of specific antibodies. Immunoglobulin production is tested before tissue culture supernatants of hybridomas producing anti-melanoma MAb are distributed to investigators within and outside the program project. MAb are purified from tissue culture supernatants and ascitic fluids of hybridoma-bearing mice. Overall, this Core will continue to provide efficient and high quality service to the participating laboratories.

View original record on NIH RePORTER →