Elucidation of the interactome for the bioactive disaccharide Man(B1-4)GlcNAc

Scripps Research Institute, The, La Jolla CA

Investigators

Abstract

PROJECT SUMMARY Some autoimmune diseases, including systemic lupus erythematosus or “lupus,” are now known to occur because of the pathogenic excess of an intracellular disaccharide called Man(b1- 4)GlcNAc. The accumulation of this disaccharide in cells can lead to the erroneous activation of immune responses, leading to autoimmunity. The molecular mechanisms through which Man(b1- 4)GlcNAc regulate autoimmunity remain unknown, due to the difficulties of capturing the intracellular interactors and receptors of free oligosaccharides, like Man(b1-4)GlcNAc. We will use a chemoproteomic mass spectrometry-based approach to elucidate the interactome of Man(b1-4)GlcNAc to identify its functional receptors. The accomplishments resulting from this work will result in an enhanced understanding of aberrant glycosylation and the bioactivity of free Man(b1-4)GlcNAc, as well as pave the pathway for studying the interactomes of intracellular free oligosaccharides.