Viral Production Core Facility

National Institute Of Neurological Disorders And Stroke

Investigators

Abstract

In FY 2023, the Viral Production Core Facility initiated 44 projects for producing 63 AAV and lentivirus viral vectors; 50% of the projects were for NINDS labs. The other projects were for laboratories in NIMH, NCI, and NIAAA. These multi-institutional projects of particular scientific significance included: 1) design and construction by the core of an AAV capable of expressing protein and shRNA from the same viral construct for an NINDS laboratory; 2) production of a second-generation rabies viral vector for retrograde targeting of projection neurons for a laboratory in NIAAA; 3) design and construction by the core of INTRSECT expressing AAVs for an NIMH laboratory; 4) production of lentiviral vectors for screening CAR-T constructs for labs in NCI. The service the core facility provided to NINDS/NIH labs beyond essential viral vector production also included consultations on the use of viral vectors and assistance in research and design of projects for specific targeting by viral vectors.