Development of NHP Models for Infectious Pathogens of Man

National Institute Of Allergy And Infectious Diseases

Investigators

Linked publications & trials

Abstract

For norovirus, we have begun the process of collecting norovirus strains from humans, and we'll mix them together in a single challenge to multiple nonhuman primates to try to find one or more strains that show at least some minimal replications in the primate gut. We've also successfully begun to grow nonhuman primate enteroids that shows the right morphology and cell composition. In collaboration with Dr Kwong at the VRC come on we will be using his NV probes to identify antigen specific B cells in both humans and in NHP, we from which we can generate novel monoclonal antibodies. For WEVEE, we have isolated almost 100 distinct nonhuman primate monoclonal antibodies two the three viruses; with our methodology, we can selectively identify those which are cross reactive to all three. We have shown that antibodies which bind all three viruses, will only neutralize one -- IE, neutralizing epitopes on the surface of these viruses are distinct. This suggests that Monoclonal antibody therapeutics or preventatives may require at least two distinct monoclonal antibodies for protection against all three viruses. In collaboration with Dr. Kwong at the VRC, we are crystalizing these monoclonal antibodies in context of each of the viral spikes in order to define the complete antigenic surface structure of the viruses and understand why they have distinct neutralizing epitopes.