FDA Vet-LIRN Capacity-Building of Ohio ADDL to support bacterial identification of foodborne pathogens

Ohio State Department Of Agriculture, Reynoldsburg OH

Investigators

Abstract

This proposal from the Ohio Department of Agriculture (ODA) is in response to Funding Opportunity Announcement (FOA) PAR-18-604 from the Food and Drug Administration (FDA) Center for Veterinary Medicine (CVM) Veterinary Laboratory Investigation and Response Network (Vet-LIRN). This proposal from ODA’s Animal Disease Diagnostic Laboratory (ADDL) addresses the following key project areas: 1. Purchase of equipment needed to improve diagnostic capacity for Vet-LIRN case investigations of foodborne pathogens. a. We are requesting funds to support to the purchase of a Bruker MALDI Biotyper® Sirius RUO System for bacterial identification. 2. Conduct increased testing in response to a new need identified by the VPO. a. MALDI-TOF technology allows for high throughput identification of bacterial isolates to reduce turnaround time of bacterial culture and isolation. Additionally, participation in additional projects such as method development and/or method validation/improvement and matrix extension work as determined by the VPO may be included in the capacity-building funds. This proposal will maintain the capacity of our laboratory to conduct molecular diagnostic testing for high-throughput sample processing capability and data analysis by maintaining active service contracts to reduce any delays in testing due to equipment malfunctions or needed repairs.