Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution

$22,995R35FY2023GMNIH

University Of Virginia, Charlottesville VA

Investigators

Linked publications & trials

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Abstract

Abstract Supplementary funds are requested to purchase a glow discharge apparatus to be used nearly daily for the projects funded by this R35. Glow discharge of EM grids is needed for both negative stain and cryo-EM applications to clean the grids and make them more hydrophilic. This is a very important step in sample preparation, and is crucial to getting good ice for cryo-EM. The existing apparatus was purchased in my lab ~ 13 years ago, is failing to function properly, and cannot be repaired in any cost-effective way.

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