Molecular Mechanisms Underlying Adaptor-Mediated Integrin Signaling in a Species-Specific Manner

Research Inst Of Fox Chase Can Ctr, Philadelphia PA

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Abstract

Project Summary This is a request for an NIGMS Administrative Supplement to NIGMS grant R35GM119560, to purchase a miniDAWN Multi-Angle Light Scattering (MALS) Detector from Wyatt Technology. Our previous structural and functional studies of integrin signaling pathway reveal that integrin activity is tightly regulated by a few signaling adaptor proteins. Formation of protein complexes or protein homo-oligomers mediates the signal transduction that leads to integrin activation and clustering. With the miniDAWN MALS detector, we can monitor the formation of these complexes/oligomers by accurately determining the absolute molar masses and sizes of protein macromolecules in solution. The instrument will provide the necessary capabilities to maximize productivity and fully achieve the aims of the proposed research project. Institutional matching funds will contribute to the purchase of this instrument.