Cytek Aurora CS

Vanderbilt University Medical Center, Nashville TN

Investigators

Abstract

PROJECT SUMMARY/ABSTRACT Biomedical research is undergoing a revolution in single cell biology. Our ability to examine the proteome and genome at the single cell level has never been greater. Thus, we have never had a greater need to isolate and purify rare stem and progenitor cells, maturing organ-specific mesenchymal or epithelial cells, or novel immune subsets. Long awaited computational advancements to fluorescence activated cell sorting now allow us to capture and deconvolute the entire fluorescent spectrum of a fluorophore. This technology is revolutionizing our ability to purify single rare cells. The instrument we are requesting is a Cytek Aurora CS and represents a major breakthrough in flow cytometry that will remove the 8-12 color barrier that scientists have struggled with due to bleed over of the spectra. This instrument is also intended as a supplement for aging instrumentation that is close to the end of its service life and used beyond its expected capacity. The Aurora CS uses 64 channels of fluorescent detection to sort single cells. This new technology will bring new challenges and still require robust quality control protocols and knowledgeable professional personnel with a deep understanding of the electronics, physics, and biology required to maximize this instrument’s usage and potential. The Vanderbilt Flow Cytometry Shared Resource (VFCSR) is optimally configured to implement this instrument successfully and serves a broad group of NIH funded scientists whose research Aims are dependent on flow cytometry measurements. Of key importance, common fluorescent reagents, already used currently on our Cytek Aurora, can be used on this platform. Custom reagents are not required, yet are still compatible. This instrument is capable of performing all levels of cell sorting, from basic sorts to the upper limit of high-content flow cytometry. New reagents are in development to maximize this instrument’s potential, currently estimated to be 45 color panels. VUMC maintains an active administration and support infrastructure for the grant-funded projects of its investigators totaling nearly $460M in research funding annually, and contributing to the majority of the School of Medicine NIH funding. This instrument will greatly benefit investigators that require cell sorting while conducting cutting-edge research on cancer, digestive diseases, immunology, infection biology, and immunotherapy. This instrument also will enhance our ability to resolve and sort small particles like cellular vesicles and worm neurons, allowing us to better serve our diverse research base in the region. The addition of this sorter, with its great flexibility and fluorescent-parameter potential will be very impactful for Vanderbilt scientists. The capability to generate more valuable data per sample and per cell, will enhance the ability of the Vanderbilt research community to make break-through discoveries for the advancement of science and medicine.