In vivo Multimodal Wide Field of View Multiphoton Microscope for Implantable Medical Devices Research

University Of Pittsburgh At Pittsburgh, Pittsburgh PA

Investigators

Abstract

Project Summary: This request is for funds to purchase a dual-line Bruker 2pPlus multiphoton system equipped with a resonant and galvanometer-driven scan head and 4 GaAsP detectors. This instrument will be housed within the Center for Biotechnology and Bioengineering at the University of Pittsburgh School of Engineering. The mission of the Center is the development of cross-disciplinary research teams by providing laboratory space and interdisciplinary education programs towards improving the human condition, especially in disease and injury. A multiphoton microscopy is an essential tool being increasingly used by the investigators at the Center. Currently, the center has an individual Bruker Ultima IV system being utilized by multiple NIH-funded investigators. It is heavily used (90-100 hrs/wk), and cannot be upgraded. This application is requesting funds to purchase a Shared Bruker 2pPlus for all investigators at the Center as well as researchers in adjacent research facilities. The need for this instrument is demonstrated in this application with several NIH-supported projects utilizing medical devices, bioengineered technology, and other intervention strategies. To promote cost- effectiveness in maintenance and upgrades, optimize sharing, and foster a collaborative multidisciplinary environment, the system will be installed into a current Shared Equipment space at the Center. The Program Director, Takashi Kozai, Ph.D. is an established innovator in multiphoton microscopy and advanced neurotechnology, and rodent imaging and will take responsibility for the multiple facets of the instrument's use. An Advisory Oversight Committee has been formed with guidelines established for appropriate sharing among the users and by which new users can gain access to and training in the system. Our management scheme is designed for the optimal use of the system and maximum contribution to the listed projects and to continuously identify other NIH projects that will benefit from this system. The acquisition of the requested in vivo multiphoton imaging system will be of enormous value not only to current NIH-funded projects across the University of Pittsburgh but also to the development of future NIH projects. This is also a key focus of training and research for the newly funded NIH T32 “Bioengineering in Psychiatry”. The University of Pittsburgh's Swanson School of Engineering and Department of Bioengineering are fully committed to the support of maintenance, personnel, space, and resources required for the long-term use of the system.