Screening to identify potent and specific inhibitors of N-terminal methyltransferase (NTMT1) as chemical probes

National Center For Advancing Translational Sciences

Investigators

Abstract

The goal of this collaboration is to uncover potent and specific inhibitors for NTMT1 as a chemical probe to investigate its mechanism, cellular targets, and functions. During this period, the collaborative team utilized the previously optimized and miniaturized NTMT1 assay to conduct a quantitative high-throughput screen of several of ETB's small molecule collections. Identified hits were cherry-picked for initial validation using the primary assay, and were subsequently tested in follow-up assays to provide additional activity data.