Development of Multidimensional, Linear and Differential Ion Mobility MS Separations for Middle-Down Proteoforms

Florida International University, Miami FL

Investigators

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Abstract

Project Summary PI: Francisco Fernandez-Lima This equipment supplement application requests funds to purchase a LEAP HDX Base System, and corresponding HDExaminer Data Analysis Software to support instrumental advances in tandem ion mobility ? mass spectrometry (IMS/IMS-MS/MS) with the goal of providing needed complementary structural information to the middle-down and top-down strategies developed for the structural characterization of DNA binding proteins and ligand interaction dynamics under the aims of the PI?s NIGMS active grant R01-GM134247. This equipment supplement request will also streamline the application of solution HDX technology in tandem with unique middle- down and top-down IMS- MS strategies for several active-funded NIGMS investigators at FIU via the Advance Mass Spectrometry Facility, directed by the PI.