BIACORE 3000

University Of Tennessee Health Sci Ctr, Memphis TN

Investigators

Abstract

Surface plasmon resonance biosensors have become important tools for measuring the binding affinities and kinetic constants of reversible interactions between molecules. BIACORE, a novel analytical system, uses surface plasmon resonance detection to rapidly determine the affinity and kinetics of ligand-receptor interactions, macromolecular complex formations, DNA protein binding and other interactions between biological macromolecules. A group of NIH-supported investigators at the University of Tennessee, Memphis campus have active research programs in the areas of cell adhesion, drug discovery, signal transduction, DNA protein binding and structure-function analysis. A BIACORE surface plasmon resonance biosensor is needed to study the reversible interactions between biological molecules where increased resolution in kinetic analysis is essential, increased sensitivity is required and for mufti-sample analysis through automation that will facilitate experimentation and save time. We are requesting a BIACORE 3000 to enhance our research activity and productivity and to assist us in accomplishing the goals of our funded NIH grants.