DNA-decoded Libraries of Reversible, Covalent Protein Ligands

Deluge Biotechnologies, Inc., Jupiter FL

Investigators

Abstract

Project Summary There is increasing interest in the development of covalent ligands of proteins, which have many advantages over non-covalent ligands. The goal of this proposal is to develop large libraries of DNA-encoded libraries of potential covalent ligands and to optimize methods to screen them. This would represent a major advance over current technology to identify covalent ligands, which almost always relies on structure-aided grafting of an electrophile onto an existing reversible ligand. The successful completion of this Phase I effort would establish a platform that Deluge could offer customers in the form of a custom screening service for the identification of potent and selective covalent ligands for any protein of interest to the customer that has surface-accessible cysteines.