qHTS to Identify Inhibitors of Zika Virus

National Center For Advancing Translational Sciences

Investigators

Abstract

During this period, NCGC and the collaborative team worked to screen approximately 6,000 compounds mostly (derived from natural products). A miniaturized high-throughput screening assay was developed to detect Zika virus (Ugandan 766) infection, which causes a viral cytopathic effect (CPE) in Vero cells. CPE inhibition was measured by viability assay in a miniaturized 1536-well qHTS assay format. Hit compounds were identified and cherry-picked for further testing in Dengue, West Nile and Yellow Fever flavivirus assays to determine selectivity.