Craniofacial Tissue Engineering

$506,658R01FY2016DENIH

Columbia University Health Sciences, New York NY

Investigators

Linked publications & trials

Paper 33434713 Paper 31844276 Paper 31626302 Paper 30505261 Paper 30260527 Paper 30140193 Paper 29499151 Paper 29415631 Paper 29363599 Paper 29040875 Paper 28843732 Paper 28600846 Paper 28407495 Paper 28228529 Paper 28193145 Paper 28068876 Paper 27940389 Paper 27931263 Paper 27912680 Paper 27481330 Paper 27306665 Paper 27255605 Paper 27236665 Paper 27198889 Paper 27115504 Paper 27089917 Paper 26914869 Paper 26714410 Paper 26599624 Paper 26195329 Paper 25828645 Paper 25787736 Paper 25618295 Paper 25526931 Paper 25458579 Paper 25453950 Paper 25394295 Paper 25331098 Paper 25307081 Paper 25281788 Paper 24881027 Paper 24778247 Paper 24610728 Paper 24419206 Paper 24281874 Paper 24014312 Paper 23653480 Paper 23651296 Paper 23588785 Paper 23569284 Paper 23531529 Paper 23372533 Paper 23203946 Paper 23197642 Paper 22903929 Paper 22901965 Paper 22586099 Paper 22421311 Paper 22147066 Paper 22016073 Paper 21856057 Paper 21774080 Paper 21449028 Paper 21362565 Paper 21291478 Paper 21262535 Paper 21196358 Paper 20924519 Paper 20725950 Paper 20637059 Paper 20175974 Paper 19795385 Paper 19678762 Paper 19198002 Paper 19146967 Paper 19075755 Paper 18952171 Paper 18940727 Paper 18822398 Paper 18718655 Paper 18694293 Paper 18637024 Paper 18620487 Paper 18299159 Paper 18230586 Paper 17092555 Paper 16895736

Abstract

DESCRIPTION (provided by applicant): Regeneration of normal shape, architecture and function of craniofacial tissues due to congenital abnormality, trauma or surgical treatment presents special problems to tissue engineering. Because of the great variations in properties of these tissues, currently available treatment options fall short of adequate care. The availability of customized living tissues engineered in vitro would revolutionize the way we currently treat craniofacial defects. In the 1st grant cycle, we established a tissue engineering approach to craniofacial reconstruction based on anatomically shaped cartilage/bone grafts formed using human stem cells, composite scaffolds and perfusion/loading bioreactors. From these studies, we published 74 peer-reviewed journal articles, 27 of which were coauthored by the two laboratories (Columbia University and Tufts University). For the 2nd grant cycle, we will maintain our focus on the temporomandibular joint (TMJ) and build upon the results of the 1st cycle to make advances in three important areas: (i) Develop composite scaffolds mimicking the internal architectures of craniofacial tissues, (ii) Integrate bioreactors with live imaging to study tissue development without interrupting cultivation, and (iii) Conduct a large animal study of craniofacial reconstruction. The research plan has been designed for impact in two areas: (i) Customized approach to craniofacial reconstruction, and (ii) Quantitative insights into the formation of craniofacial tissues, using an advanced bioreactor-imaging system and a clinically relevant animal model. Our overall hypothesis is that craniofacial grafts can be formed by biophysical regulation of adult human stem cells using a biomimetic scaffold-bioreactor system. We will engineer the TMJ condyle and the TMJ disc, two important and interacting jaw components, with complex architectures and mechanical loading, but with distinct structures. Three specific aims will be pursued: Aim 1 - Develop anatomically shaped scaffolds for engineering TMJ condyle and disc, with their characteristic structural and biomechanical anisotropies, Aim 2 - Engineer customized craniofacial tissues using an advanced bioreactor-imaging platform, and Aim 3 - Evaluate the tissue engineering approach to craniofacial reconstruction in a large animal model (Yucatan pig).

View original record on NIH RePORTER →