LABELING OF ADENOVIRUS 12 KNOB
$293,069P41FY2001RRNIH
Brookhaven Science Assoc-Brookhaven Lab, Upton NY
Investigators
Linked publications & trials
Paper 25242887Paper 24793749Paper 20887859Paper 20155329Paper 18814851Paper 18639797Paper 18154334Paper 18006331Paper 17688395Paper 17548765Paper 16203986Paper 15567868Paper 15208690Paper 14557074Paper 12972252Paper 12498796Paper 12379108Paper 12367530Paper 12064938Paper 12008948Paper 11851882Paper 11703654Paper 11562162Paper 11357616Paper 11290566Paper 11278978Paper 11222712Paper 11222698Paper 11170476Paper 11148208Paper 10908577Paper 10677495
Abstract
The adenovirus protein, A12, is involved in the binding of the virus to the receptor. The protein has been cloned with 6-histidine tail for purification and a thrombin site to remove the his-tail. A NiNTA-Nanogold probe was synthesized and tested with this protein, which is a trimer of 64 kDa (a good STEM sample). The thrombin-cleaved protein was used as a control. Nearly every protein with the his-tail was labeled, often with 2-3 gold clusters, consistent with it being a trimer. By STEM, the non-his protein (cleaved) showed no cluster binding.
View original record on NIH RePORTER →