XRAY SCATTERING ON MG2+ PROMOTED STRUCT CHANGE OF ENDO &EXONUCLEASE FEN 1

$143,176P41FY2001RRNIH

Stanford University, Stanford CA

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Abstract

This 42 kDa endo-/exonuclease (flap endonuclease and 5'-exonuclease) FEN-1 is important in DNA replication and damage repair. We conducted preliminary solution scattering experiments in the presence and absence of Mg2+ on the wild type FEN-1 and a site-directed mutant whose repair function is inactivated. Although the high-flux synchrotron beam allowed us to study these proteins at fairly low concentrations (as low as 1 mg/ml), our preliminary results suggest that the proteins needs to be measured at a rather low temperature (around 5 0C) instead of 15 0C where most of the preliminary measurements were done this time. Further studies will be performed by submitting a separate experimental proposal. Parallel neutron scattering studies are in progress.

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