EXXON PRT TIME SULFUR K EDGE SPECT IN SITU PROBE OF SULFUR SPECIES IN BACTERIA

$143,176P41FY2001RRNIH

Stanford University, Stanford CA

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Abstract

Microbial desulfurization offers a potential new approach to removing sulfur compounds from fuel oils with minimal environmental impacts (Gray et al., (1996) Nature Biotechnology 14, 1705-1709). Progress has been made in isolating the genes for the individual enzymes in the desulfurization pathway, and studying their function in recombinant E. coli. We are using sulfur K-edge spectroscopy to follow the desulfurization of fuel oils by these organisms in an attempt to understand their substrate specificity in complex mixtures.

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