HIGH RESOLUTION DATA COLLECTION OF LARGE OLIGOMERIC PROTEINS

$143,176P41FY2001RRNIH

Stanford University, Stanford CA

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Abstract

We propose to collect diffraction data on four projects from our laboratory: ornithine decarboxylase (mODC) from mouse, biosynthetic arginine decarboxylase (bADC) from Escherichia coli (E. coli), the truncated (yeast tE2) components of the pyruvate dehydrogenase complex from Sacchromyces cerevisiae, and the tE2 from the a-ketoglutarate dehydrogenase complex from E. Coli. Data collection of a select group of heavy metal derivatives for both mODC and bADC would aid in the structural determination of these two enzymes.

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