LOW RESOLUTION DATA COLLECTION OF YEAST RNA POLYMERASE II (POL II)

$143,176P41FY2001RRNIH

Stanford University, Stanford CA

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Abstract

We have recently obtained high resolution three dimensional crystals of Pol II at atomic resolution diffraction. We are now pursuing modified crystals, including a tight binding inhibitor and cocomplexes with DNA and DNA/RNA ternary complexes as simple routes to heavy atom derivatives. We have improved our crystals from an earlier limit of 30 E for unfrozen crystals to better than 3.5 E on our recent run using flash freezing and are attempting to improve the purity of the protein for lower mosaicity. Simultaneously, we are attempting to obtain the lowest resolution reflections for a direct phasing of the protein.

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