NUN-RNA INTERACTIONS

$0P41FY2001RRNIH

Rockefeller University, New York NY

Investigators

Linked publications & trials

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Abstract

The NUN protein is expressed by E-coli after infection with lambdoid phage HK022. The presence of the protein terminates the transcription of DNA and prevents superinfection of E-coli by other lambda phages. From a tryptic digest of the NUN-RNA complex it was determined using MALDI-TOF mass spectrometry that the interaction region was traced to a 30 residue stretch of the protein. More detailed mapping of the binding region is being carried out through the use of a ladder of peptides that span the region of interest. At the same time, the NUN-RNA interaction is under intense study using multidimensional NMR spectroscopy.

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