Core B: Cell Banking and Immune Assessment Core

$262,387P01FY2015CANIH

Dana-Farber Cancer Inst, Boston MA

Investigators

Linked publications & trials

Paper 36459673 Paper 36189229 Paper 35967386 Paper 35475885 Paper 35363464 Paper 35226736 Paper 34712243 Paper 34631716 Paper 34575843 Paper 34570880 Paper 34400321 Paper 34291733 Paper 34265047 Paper 34217703 Paper 34101618 Paper 34006823 Paper 33877965 Paper 33877856 Paper 33855972 Paper 33666654 Paper 33428454 Paper 33421294 Paper 33045061 Paper 31697815 Paper 31648315 Paper 31471324 Paper 31285436 Paper 31253630 Paper 31243040 Paper 30936059 Paper 30936057 Paper 30748099 Paper 30655371 Paper 30568034 Paper 30537514 Paper 30426537 Paper 30392958 Paper 30279226 Paper 30228128 Paper 30181175 Paper 30171221 Paper 30108109 Paper 30061358 Paper 30006305 Paper 29728401 Paper 29530952 Paper 29459719 Paper 29445371 Paper 29437591 Paper 29348127 Paper 29326124 Paper 29296875 Paper 29202477 Paper 29197137 Paper 29171820 Paper 29138220 Paper 29093061 Paper 28941323 Paper 28931653 Paper 28924018 Paper 28851699 Paper 28665299 Paper 28652577 Paper 28614794 Paper 28550042 Paper 28487567 Paper 28470889 Paper 28416503 Paper 28254742 Paper 28151427 Paper 28073785 Paper 27887864 Paper 27821505 Paper 27821504 Paper 27812545 Paper 27777141 Paper 27711974 Paper 27699243 Paper 27630126 Paper 27590106 Paper 27485827 Paper 27436361 Paper 27385791 Paper 27354023 Paper 27323847 Paper 27312177 Paper 27222477 Paper 27195312 Paper 27160197 Paper 27135738 Paper 27112509 Paper 27073224 Paper 26994149 Paper 26983850 Paper 26944544 Paper 26883876 Paper 26837700 Paper 26806586 Paper 26729448 Paper 26670634

Abstract

Project Summary/Abstract Core B The purpose of this core is to provide a centralized laboratory resource for obtaining, cryopreserving and distributing patient samples and to monitor the kinetics of immune reconstitution following allogeneic hematopoietic stem cell transplantation (HCT). Prior to transplantation, peripheral blood mononuclear cells (PBMC), bone marrow and serum or plasma are obtained from all patients enrolled on clinical trials supported by this Program Project. PBMC, plasma and serum are also obtained from normal stem cell donors for these individuals. Following HCT, peripheral blood, plasma and DNA samples are obtained at regular intervals. All of these samples are processed, cryopreserved, entered into a computerized inventory and made available to all of the investigators in this program. To support clinical trials in Project 1, the effects of immunologic interventions are evaluated through serial assessment of specific lymphocyte populations that define changes in number as well as their level of activation, maturation and other functional characteristics. Phenotypic analysis of circulating lymphocytes primarily utilizes multi-parameter flow cytometry with a panel of fluorochrome-conjugated monoclonal antibodies. Plasma concentrations of cytokines known to play important roles in immune reconstitution (BAFF, IL-2) are measured by ELISA. Reconstitution of B and T cell receptor repertoire is examined by in depth sequencing of immunoglobulin and TCRV genes. Thymic function is evaluated by quantitative PCR measurement of T-cell receptor excision circles (TREC). Reconstitution of T cell immunity to specific target antigens such as CMV and EBV is determined by ELISPOT and HLA-tetramers. In Project 3, patient plasma is used to identify antibodies specific for HY antigens or other allo or auto antigens. Results of each of these assays are correlated with other parameters of immune function as well as with clinical outcomes.

View original record on NIH RePORTER →