Cryoelectron microscopy images for molecular replacement

$0P01FY2001GMNIH

Brandeis University, Waltham MA

Investigators

Linked publications & trials

Paper 32004465 Paper 29453354 Paper 28538735 Paper 28506635 Paper 27881300 Paper 27667691 Paper 27501074 Paper 27434674 Paper 27428774 Paper 27428773 Paper 27223329 Paper 27185936 Paper 27159452 Paper 26356202 Paper 26351699 Paper 26318383 Paper 26144317 Paper 25910204 Paper 25635393 Paper 25383525 Paper 25157102 Paper 25092336 Paper 25043550 Paper 24927574 Paper 24916797 Paper 24906155 Paper 24821769 Paper 24657230 Paper 24560806 Paper 24324137 Paper 24189638 Paper 23872434 Paper 23674680 Paper 23294159 Paper 23136972 Paper 23089332 Paper 23084398 Paper 23082933 Paper 23022349 Paper 23000701 Paper 22613568 Paper 22609687 Paper 22366277 Paper 21757012 Paper 21752659 Paper 21681963 Paper 21435344 Paper 21411326 Paper 21333526 Paper 21157433 Paper 21098306 Paper 20972447 Paper 20888965 Paper 20888471 Paper 20887861 Paper 20887857 Paper 20887854 Paper 20562026 Paper 20558298 Paper 20538058 Paper 20479883 Paper 20308582 Paper 20181687 Paper 20069616 Paper 20033059 Paper 20032458 Paper 19933700 Paper 19843697 Paper 19718020 Paper 19643814 Paper 19597329 Paper 19489732 Paper 19487668 Paper 19416061 Paper 19402748 Paper 19361419 Paper 19258014 Paper 19208805 Paper 19038266 Paper 18775332 Paper 18655791 Paper 18484707 Paper 18483195 Paper 18441012 Paper 18397883 Paper 18347330 Paper 18294654 Paper 18252254 Paper 18238898 Paper 18194855 Paper 18166196 Paper 18082611 Paper 17977018 Paper 17964808 Paper 17723294 Paper 17692533 Paper 17638580 Paper 17599066 Paper 17585939 Paper 17535912

Abstract

The principal goal of this project is to develop uses of images derived from electron cryomicroscopy as molecular replacement models for x-ray crystallography. A second goal is to use high-resolution structures of viral proteins and subviral particles, together with cryoEM reconstructions of intact virions, to obtain detailed models for complex viruses. (1) Human papillomavirus 16 (HPV16) small virus-like particles (VLPs) will be used as test objects, to work out the best ways of determining the orientation and position parameters for placing a 3-D image reconstruction in the unit cell of a crystal and to analyze the resulting phase correlations in different resolution shells. We will analyze the effects of CTF corrections, procedures for deriving the reconstructed image, and molecular- replacement algorithms on the phase-determining power of the model. (2) Molecular replacement approaches will be used to solve the phase problem for rotavirus inner capsid particles (ICPs), for the rotavirus outer-shell protein, VP7, and for the retrovirus" penetration complex", mu13SIGMA3/3. 93) Crystal structures for components of retrovirus and rotavirus particles will be used, together with EM reconstructions of the intact viruses, to build models for the intact virions.

View original record on NIH RePORTER →