Ubiquitin signaling

Division Of Basic Sciences - Nci

Investigators

Linked publications, trials & patents

Abstract

We have moved all aspects of this umbrella project forward during 2013. We discovered that C-terminal domains within ATPase proteins that dock against the proteasome's core particle undergo dynamic exchange between an expected 4-helix bundle and a partially unfolded state. We provided evidence that this exchange is important for interactions with chaperones involved in proteasome assembly. In unpublished work, we have moved forward the resolution of the 3D structures for multiple protein complexes, including a new ubiquitin binding motif. During 2013, we also characterized a small molecule that restricts proliferation of multiple cancer cell lines. This work was collaborative with the Roden lab at Johns Hopkins University and led to the characterization of ubiquitin receptor Rpn13 as a potential target for certain cancer types.