Presynaptic Mechanisms in Dopamine Neurotransmission

Columbia University Health Sciences, New York NY

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Abstract

DESCRIPTION (provided by applicant): The importance of ventral midbrain dopamine (DA) synaptic activity is clear from its roles in motor and behavioral disorders, including drug dependence. These synapses contribute to the normal execution of motor sequences, learning, and habit formation by mediating short- and long-term plasticity in the basal ganglia. DA neuronal activity has been difficult to characterize by conventional technologies since the axons are extremely long and complex and do not make conventional ionotropic synapses that can be recorded by electrophysiology. We have thus developed new optical techniques, including fluorescent false neurotransmitters (FFNs) that provide the first means to examine neurotransmission at the level of individual synapses: the first two publications were part of the current grant cycle, and the requested continuation will introduce second generation FFNs that have important advantages over the first. In this work, we will first determine the signal by which DA axons grow and make synapses at the correct positions in the striatum: this appears to be triggered by glutamate inputs, apparently either from cortical inputs or from DA neurons themselves. These experiments will use one of the new FFNs, the first to work in neuronal cultures. Then we will identify the signals that make these DA synapses active: our Preliminary Results indicate that most DA terminals are silent and do not normally release neurotransmitter, but that this is modulated by a newly identified form of synaptic plasticity. Thi will use another new FFN that is well adapted for measuring DA transmission simultaneously with the synaptic vesicle fusion probe FM1-43. Finally, we will examine how DA and its loss in a unilateral Parkinson's disease model leads to selection of the correct corticostriatal synapses that underlie simple motor behaviors in the mouse, i.e., paw use to explore a novel environment, and a feeding behavior. To our knowledge, this will be the first time that particular synapses within a neuronal projection would be identified to underlie a behavior in the vertebrate CNS.