Progenitor Cell Analysis Core

$92,193P30FY2014GMNIH

Mainehealth, Portland ME

Investigators

Linked publications & trials

Paper 38991122 Paper 38553695 Paper 38335918 Paper 38325139 Paper 38040222 Paper 37946877 Paper 37560296 Paper 36581316 Paper 36166296 Paper 36054037 Paper 35986566 Paper 35609807 Paper 35226161 Paper 35122666 Paper 34789851 Paper 34714716 Paper 33436016 Paper 33359894 Paper 33259856 Paper 33210065 Paper 32972203 Paper 32845528 Paper 32814099 Paper 32786095 Paper 32473295 Paper 32348021 Paper 32336178 Paper 32259549 Paper 32251632 Paper 32199337 Paper 32154009 Paper 32133436 Paper 32027948 Paper 31874253 Paper 31621880 Paper 31561084 Paper 31266807 Paper 31170511 Paper 31101754 Paper 30954729 Paper 30953371 Paper 30941770 Paper 30805994 Paper 30742263 Paper 30729461 Paper 30540952 Paper 30356120 Paper 30354669 Paper 30118657 Paper 30105417 Paper 30085049 Paper 29897433 Paper 29765356 Paper 29756030 Paper 29667914 Paper 29440419 Paper 29377150 Paper 29366838 Paper 29342317 Paper 29326589 Paper 29291395 Paper 29196442 Paper 29105817 Paper 29058774 Paper 28977604 Paper 28938423 Paper 28889371 Paper 28821457 Paper 28715127 Paper 28689816 Paper 28631813 Paper 28580233 Paper 28538730 Paper 28515094 Paper 28460416 Paper 28432594 Paper 28432589 Paper 28408238 Paper 28235789 Paper 28206683 Paper 28184001 Paper 28162969 Paper 28131135 Paper 28062363 Paper 27911598 Paper 27790370 Paper 27638773 Paper 27430622 Paper 27378017 Paper 27343063 Paper 27302072 Paper 27254003 Paper 27174804 Paper 27084391 Paper 27033451 Paper 27002787 Paper 26976794 Paper 26973433 Paper 26950413 Paper 26910604

Abstract

PROJECT SUMMARY (See Instructions): Towards advancing Center and Institute investigations (and continued Center development), our Progenitor Cell Analysis Core provides expert services, training, instrumentation and resources in three related areas: i) analytical flow cytometry, together with FACS and MACS cell isolation services; ii) high through-put colony/cell imaging, fluorimetry, and luminometry; iii) services/resources for stem & progenitor cell culture, including unique cell populations, plus ES cells and approved cell lines. Several core supported services (eg, flow cytometry and FACS) are indispensable, unique components, and are used widely by Center and Institute investigators. Combined instrumentation as a BD FacsCalibur flow cytometer plus FACSAria-2 cell sorter (near-UV laser) enable complex four color multi-parameter analyses. This includes cell surface markers, as well as (co-) analyses of cell cycle parameters, apoptosis, phosphoprotein signaling, ROS, calcium flux and a number of additional informative cell properties. Our expert staff are also skilled in the enrichment and isolation of rare cell sub-populations (eg, using combined MACS plus FACS approaches). In addition, this core supports basic analyses (and manipulations of) progenitor cell populations (Vicell system, injectable multi-well luminometer, fluorimeter, electroporation/transfection units). Recently, we also have launched several innovative cell-based analyses, with cell and colony scanning via STEMvision as one example. With regards to services and resources for the culture and manipulation of stem and progenitor cells, these benefit further from a dedicated tissue culture facility (Zeiss and Evos fluorescent microscopy systems, cell culture hoods and incubators, hypoxia chambers, clinical centrifuges, specialty reagents). Our Progenitor Cell Analysis Core also interacts directly with Center and Institute cores in Histopathology, Molecular Phenotyping, Confocal Microscopy and Viral Vectors; facilitates productive multi-unit interactions; provides vital roles in new Center and Institute faculty recruitment; and continues to advance our biomedical research investigations (together with nationally competitive grant applications).

View original record on NIH RePORTER →