2012-Directed Differentiation of Stem Cells to Cardiomyocytes Using Optically Act

Massachusetts Institute Of Technology, Cambridge MA

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Abstract

DESCRIPTION (provided by applicant): We propose a new technology for dynamic stem cell culture, using arrays of optically actuated polymer microposts. The polymer structures will be created by replica molding of straight and slanted microposts, made using carbon nanotube (CNT) and silicon master molds. The replica structures will be cast using photoactive liquid crystal elastomers (LCEs). Illumination of the LCE microposts will cause a rapid change in their shape and/or stiffness; and it will be possible to actuate arbitrary areas of posts based on the spot size and intensity of the illumination beam. We will assess cell viability on LCE structures i comparison to conventional poly- dimethylsiloxane (PDMS) structures as a benchmark material, by engineering the geometry and stiffness of the two materials. Using designed arrays of microposts enabling dynamic modulation of rigidity and strain that mimics the amplitude and force of cardiomyocyte beating, we will investigate pathways for mechanically-modulated differentiation of human embryonic stem cells (hESCs) into cardiomyocytes. Thus, we will aim to improve the accuracy and throughput of cardiomyocyte differentiation, and the understanding of how mechanotransduction affects cardiogenic differentiation. Successful development of this novel system would enable further research on mechanotransductive signaling under dynamic control, and possibly the realization of mechanically controlled assays for high-throughput cell culture. 1