GSK3b mediates radiation-induced cytotoxicity in hippocampal neurons

Ohio State University, Columbus OH

Investigators

Linked publications & trials

Abstract

DESCRIPTION (provided by applicant): A formidable challenge in the treatment of primary and metastatic brain cancers, especially in children, is the long-term neurocognitive deficiencies resulting from cranial irradiation (IR)-induced hippocampal neuronal apoptosis. Our laboratory has discovered a novel functional connection between the metabolic kinase GSK3¿ and the Non- homologous End-joining (NHEJ) pathway that repair DNA double-strand breaks (DSBs). Furthermore, our preliminary data revealed that the NHEJ mediator 53BP1 is directly phosphorylated by GSK3¿; meanwhile, increased expression of the classic GSK3¿ substrate ¿-catenin is associated with enhanced repair of IR-induced DSBs and survival in hippocampal neurons. Thus, we hypothesize that GSK3¿ regulates NHEJ-mediated repair of DSBs and determines neuron cytotoxicity following IR via suppression of 53BP1 and ¿-catenin function. In addition, tumor cells which contain abnormal GSK3 ¿ activity will not exhibit GSK3¿-mediated protection from IR-induced cytotoxicity. A series of in vitro and in vivo experiments are proposed to test our hypotheses: Aim 1 will identify the GSK3 ¿ phosphorylation sites in 53BP1 and determine whether GSK3 ¿ -specific phosphorylation direct 53BP1 function in NHEJ and in survival of irradiated hippocampal neurons. Aim 2 will determine whether GSK3 ¿ regulates 53BP1 through suppressing ¿ - catenin that may promote NHEJ activity by increasing 53BP1 transcription, or by directly interacts with 53BP1. Aim 3 will determine if abnormal GSK3 ¿ activity determine brain tumor cell resistance to the prophylactic GSK3 ¿ -inhibition mediated protection from radiation induced cytotoxicity.