Nucleosome Dynamics

Division Of Basic Sciences - Nci

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Abstract

For FY12, we have used multi-dimensional NMR and site-directed mutagenesis methods to assign the chemical shifts of methyl groups of Val, Leu, and Ile of histones in the nucleosome. They were used as probes for studying the dynamics of nucleosome and its interactions with other proteins such as linker histone H1 and high mobility group nucleosomal proteins (HMGN), kinetochore protein CENP-C. We have determined the structure of the HMGN2-nucleosome complex. We have also investigated the structure of Scm3 complexed with centromere histone variant Cse4 and histone H4. Our studies are important for understanding how chromosomes are segregated during cell division.