Flow Cytometry

$151,413P30FY2012CANIH

Wistar Institute, Philadelphia PA

Investigators

Linked publications & trials

Paper 39622583 Paper 39592860 Paper 39579354 Paper 39570374 Paper 39563034 Paper 39539277 Paper 39520074 Paper 39500865 Paper 39481645 Paper 39386512 Paper 39314469 Paper 39282282 Paper 39265628 Paper 39258908 Paper 39243764 Paper 39241017 Paper 39225484 Paper 39222637 Paper 39213189 Paper 39191802 Paper 39163336 Paper 39120311 Paper 39109927 Paper 39105261 Paper 39091739 Paper 39082680 Paper 39073185 Paper 39043790 Paper 39010681 Paper 39008664 Paper 39007351 Paper 39005383 Paper 38987289 Paper 38979222 Paper 38966817 Paper 38961054 Paper 38926365 Paper 38844208 Paper 38834201 Paper 38815867 Paper 38802657 Paper 38798466 Paper 38766222 Paper 38746323 Paper 38703775 Paper 38659891 Paper 38646411 Paper 38641411 Paper 38626917 Paper 38626768 Paper 38600088 Paper 38584166 Paper 38565883 Paper 38522521 Paper 38519053 Paper 38464183 Paper 38417134 Paper 38393317 Paper 38392869 Paper 38383483 Paper 38370717 Paper 38308250 Paper 38279823 Paper 38271588 Paper 38267705 Paper 38263486 Paper 38245503 Paper 38191593 Paper 38152610 Paper 38102487 Paper 38066089 Paper 38064712 Paper 38062777 Paper 38062767 Paper 37971875 Paper 37961645 Paper 37956856 Paper 37948180 Paper 37942577 Paper 37910620 Paper 37889078 Paper 37872177 Paper 37832787 Paper 37805922 Paper 37741199 Paper 37723305 Paper 37723262 Paper 37683180 Paper 37651540 Paper 37639774 Paper 37606370 Paper 37578230 Paper 37563309 Paper 37562974 Paper 37552993 Paper 37528120 Paper 37479684 Paper 37468627 Paper 37461649 Paper 37444561

Abstract

The Wistar Institute Flow Cytometry Facility provides flow cytometric services and supports the use of flow cytometric techniques by Wistar Cancer Center investigators. The Facility's aims are to:1) provide the technological capability for high quality, single and multi-parameter analyses and/or cell sorting of many types of biological cells from homogeneous or mixed cell populations;2) provide training and expertise to assist investigators in choosing experimental conditions and reagents that optimize the use of the facility's instrumentation for their experimental needs;3) advise and provide technical support for analysis of flow cytometry/cell sorting data for publication, presentation, and inclusion in grant applications, along with storing, archiving, and retrieving flow cytometric data. The laboratory houses a DakoCytomation MoFlo highspeed cell sorter, a Becton-Dickinson FACSCalibur flow cytometry system, a Cytomation CYAN-ADP Ultra- High speed 9-color analytical cytometer, a Coulter XL-MCL automated analytical cytometer, and a Becton- Dickinson FACScan Bench top Analyzer. In July 2006, the Institute purchased and placed into service a Becton-Dickinson LSR II analytical cytometer (7-color analysis), which was upgraded in November 2007 with an additional laser and detectors allowing for 10-color analysis. 24-hour access is available for all of the investigator-operated instruments. A Beckman-Coulter EPICS Elite ESP is located in the BSL3 facility, allowing for both sorting and analysis of HIV-infected samples. Also in the main facility are additional workstations, a library of flow cytometry journals, protocol guides, and other written resources. Additional equipment includes fluorescence microscopes, 4¿C refrigerators, and extensive spare parts, supplies, and tools in order to maintain the instruments at optimal operating levels. Since 2003, twenty-eight Cancer Center research groups from all Wistar research programs have utilized the Facility, generating approximately 170 publications.

View original record on NIH RePORTER →