Ethanol, Dependence and Mesolimbic Plasticity

University Of Texas At Austin, Austin TX

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Abstract

DESCRIPTION (provided by applicant): Evidence suggests that glutamate receptor trafficking induces long-term changes in the nucleus accumbens (NAc) which may constitute an important neuroadaptation contributing to the development of ethanol dependence. We have observed that low frequency conditioning stimulation (LFS) of excitatory afferents to shell NAc medium spiny neurons (msns) normally induces NMDA-receptor dependent synaptic depression in slices from ethanol na¿ve mice. However, a single 4 day bout of passive chronic intermittent ethanol (CIE) in vivo exposure induces a polarity shift in plasticity to LFS conditioning and induces synaptic potentiation (LTP) rather than LTD (metaplasticity). In addition, the shell and core subregions of the NAc likely encode different aspects of drug responding with the shell responding more to novelty and the core encoding more established learned behaviors. These findings promote our overarching hypothesis that D1-dopamine receptor-expressing medium spiny neurons of the shell and core of the nucleus accumbens differentially encode passive and operant ethanol experience through selective neuroadaptations in glutamatergic synaptic transmission and plasticity We propose in aim 1 to measure glutamatergic transmission and metaplasticity in shell/core NAc medium spiny neurons from transgenic mice expressing eGFP under the control of the dopamine D1- receptor promoter (drd1-eGFP mice) (1) after passive exposure to chronic intermittent ethanol vapor or (2) trained for volitional, operant ethanol self-administration. All experiments in aim 1 will use whole-cell voltage clamp recordings from identified drd1-eGFP shell/core msns. Alterations in glutamatergic transmission will be assessed by pharmacological isolation of NMDA and AMPA-subcomponents and AMPA:NMDA ratios. Metaplasticity will be assessed by determining the expression of LTD or LTP in response to conditioning stimulation in shell/core drd1-eGFP msns from the different ethanol experience groups. In aim 2, we will determine whether interference with GluR2 internalization alters operant self-administration behavior in mice. Injection of a peptide fragment which disrupts GluR2- mediated AMPA channel internalization will be made into the NAc to determine whether interruption of AMPAR trafficking can modulate or occlude operant responding for ethanol in the same mouse model used in aim 1. Injections will also be made into other mesocorticolimbic structures (VTA, PFC) to determine whether the behavioral effects of occlusion of LTD are limited to the NAc. These aims will advance our understanding of neuroadaptive alterations in an important mesocorticolimbic structure which may underlie the development of ethanol dependence.