MITOCHONDRIAL DNA REPAIR PROCESSES IN OXIDATIVE STRESS &

Aging

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Abstract

Summary of work: Based upon our previous finding that there is an increase with age in the activity of a mitochondrial DNA glycosylase/endonuclease (mtODE) which is specific for 8-OH deoxyguanosine (8-OHdG), we have hypothesized that there is an induction of this enzyme of DNA repair in response to chronic oxidative stress. In liver mitochondrial extracts from rats,there was a highly significant increase in activity between 6 months and 23 months of age, with the activity being at a maximum at an intermediate age (12 months). The same pattern was seen in extracts of heart mitochondria. By contrast, two other mitochondrial enzymes of DNA metabolism which are not specifically involved in the repair of oxidative damage, viz. uracil DNA glycosylase (mtUDG) and AP endonuclease, were found to be either unchanged or minimally changed with aging. This increase in mitochondrial DNA repair with aging is also seen in mice of different ages. It is surprising and may stimulate new directions as it was previously thought that DNA repair capacity declined with age. Recent experiments have used primary cultures of hepatocytes from young and old rats. We have developed new techniques to measure DNA repair in mitochondria. Using cell extracts, incision and incorporation of damaged DNA can be detected, and we can thus now approach the studies on the changes with aging more mechanistically. Repair of oxidative DNA damage is carried out by the base excision repair system. It has two pathways, long and short patch repair. Using patch size measurements we find that in human cells, oxidative lesions like uracil in DNA is repaired solely by the short patch pathway in mitochondria, while the long patch pathway is extensively used in the nuclear base excision repair.