THE BINDING PARTNERS OF ECOLI CONDENSIN MUKB

$3,911P41FY2011RRNIH

Rockefeller University, New York NY

Investigators

Linked publications & trials

Paper 33259802 Paper 33049227 Paper 32967858 Paper 32782366 Paper 32289258 Paper 31819269 Paper 31774265 Paper 27693350 Paper 27080007 Paper 26042681 Paper 25667060 Paper 25588722 Paper 25242333 Paper 25086043 Paper 24075807 Paper 23516416 Paper 23306439 Paper 23276924 Paper 23273981 Paper 22860026 Paper 22768092 Paper 22709632 Paper 22474365 Paper 22331846 Paper 22309600 Paper 22244332 Paper 22241783 Paper 22239320 Paper 22129306 Paper 22033520 Paper 22009756 Paper 21893598 Paper 21877294 Paper 21764753 Paper 21642990 Paper 21516228 Paper 21454883 Paper 21390248 Paper 21135131 Paper 21082431 Paper 21050864 Paper 20921377 Paper 20835802 Paper 20835801 Paper 20835799 Paper 20702425 Paper 20585571 Paper 20572018 Paper 20548778 Paper 20504932 Paper 20486120 Paper 20392851 Paper 20212525 Paper 20211137 Paper 20005841 Paper 19966800 Paper 19841479 Paper 19778728 Paper 19628708 Paper 19583196 Paper 19562802 Paper 19525551 Paper 19402746 Paper 19380747 Paper 19270693 Paper 19216906 Paper 19213839 Paper 19151997 Paper 19098896 Paper 18951162 Paper 18834145 Paper 18678709 Paper 18407068 Paper 18363801 Paper 18347735 Paper 18321079 Paper 18294652 Paper 18271525 Paper 18171605 Paper 18083804 Paper 17972917 Paper 17922018 Paper 17919899 Paper 17726112 Paper 17703190 Paper 17668044 Paper 17656582 Paper 17630825 Paper 17360435 Paper 17284592 Paper 17199039 Paper 17157260 Paper 17116750 Paper 16895903 Paper 16885219 Paper 16630816 Paper 16615907 Paper 16461911 Paper 16425180 Paper 16365162

Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In contrast to the current state of knowledge in the field of eukaryotic chromosome segregation, relatively little is known about the mechanisms coordinating the appropriate segregation of bacterial chromosomes. In Escherichia coli, the MukB/E/F complex and topoisomerase IV (Topo IV) are both crucial players in this process. Topo IV removes DNA entanglements following the replication of the chromosome, whereas MukB, a member of the structural maintenance of chromosomes protein family, serves as a bacterial condensin. We demonstrate here a direct physical interaction between the dimerization domain of MukB and the C-terminal domain of the ParC subunit of Topo IV. In addition, we find that MukB alters the activity of Topo IV in vitro. Finally, we isolate a MukB mutant, D692A, that is deficient in its interaction with ParC and show that this mutant fails to rescue the temperature-sensitive growth phenotype of a mukB(-) strain. These results show that MukB and Topo IV are linked physically and functionally and indicate that the activities of these proteins are not limited to chromosome segregation but likely also play a key role in the control of higher-order bacterial chromosome structure. This work was published: Li Y, Stewart NK, Berger AJ, Vos S, Schoeffler AJ, Berger JM, Chait BT, Oakley MG Escherichia coli condensin MukB stimulates topoisomerase IV activity by a direct physical interaction PNAS 107(2010)18832-7.

View original record on NIH RePORTER →