GENE EXPRESSION IN THE PRIMATE OVULATORY FOLLICLE

$3,099P51FY2011RRNIH

Oregon Health & Science University, Portland OR

Investigators

Linked publications & trials

Paper 39531811 Paper 37708918 Paper 37499736 Paper 36939178 Paper 36917864 Paper 36907435 Paper 35316218 Paper 34915175 Paper 34540718 Paper 34218053 Paper 33524104 Paper 32637610 Paper 30522845 Paper 30201462 Paper 30139430 Paper 30082858 Paper 29971796 Paper 29520866 Paper 29407362 Paper 29063622 Paper 28936565 Paper 28873045 Paper 28284896 Paper 28154169 Paper 27859771 Paper 27635049 Paper 27357159 Paper 27265023 Paper 27045379 Paper 26957355 Paper 26721679 Paper 26621857 Paper 26472457 Paper 26406934 Paper 26284599 Paper 26223349 Paper 26187412 Paper 26070857 Paper 26069734 Paper 25976012 Paper 25913366 Paper 25612682 Paper 25545382 Paper 25470211 Paper 25376878 Paper 25332397 Paper 25219362 Paper 25209798 Paper 25034695 Paper 25011649 Paper 24959820 Paper 24862904 Paper 24709587 Paper 24681059 Paper 24639226 Paper 24560476 Paper 24448900 Paper 24413616 Paper 24408325 Paper 24390280 Paper 24375078 Paper 24329418 Paper 24324408 Paper 24215535 Paper 24210020 Paper 24202052 Paper 24200973 Paper 24179112 Paper 24174435 Paper 24092271 Paper 24080959 Paper 24077067 Paper 24063855 Paper 24055980 Paper 23959334 Paper 23948905 Paper 23921100 Paper 23908248 Paper 23891857 Paper 23881606 Paper 23816568 Paper 23709130 Paper 23675327 Paper 23651991 Paper 23594602 Paper 23568382 Paper 23559626 Paper 23550738 Paper 23543038 Paper 23482462 Paper 23463525 Paper 23442156 Paper 23439920 Paper 23423613 Paper 23378731 Paper 23357537 Paper 23333375 Paper 23325800 Paper 23279544 Paper 23260193

Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In mammalian species, the midcycle surge of gonadotropin hormone (notably luteinizing hormone, LH) secreted by the pituitary gland, initiates a cascade of events in the ovary leading to ovulation of the mature follicle (i.e., rupture of the follicle wall and release of its enclosed oocyte),.. As part of the process, the gonadotropin surge acts on the preovulatory follicle to elicit cumulus-oocyte maturation, including reinitiation of meiosis in the oocyte and expansion of the surrounding cumulus cells, thereby producing a fertilizable egg that can be released from the follicle at ovulation. Rodent studies suggest that prostaglandin (PG) synthesis and local PGE2-receptor (via PGER2 not R1) action is critical for periovulatory events, including cumulus-oocyte expansion (C-OE), and fertility. Studies performed in the Stouffer laboratory demonstrated significant increases in mRNA levels for the enzymes involved in PGE synthesis, as well as PGER2, in the periovulatory follicle of rhesus macaques. Therefore, further studies were designed to evaluate whether a PGE2 antagonist, compared to vehicle control: (1) altered timely ovulation during the natural menstrual cycle, and (2) blocked C-OE in the periovulatory follicle, in rhesus monkeys. In-vivo and in vitro studies suggest that PGE2-receptor signaling promotes C-OE in primates, and provided the rationale for a new project to determine if a PGE2 antagonist was an effective, reversible contraceptive in adult, cycling monkeys.

View original record on NIH RePORTER →